Skip to content. | Skip to navigation

Personal tools
Sections
You are here: Home Programs & Services Biological Safety Additional Sites & Resources Summary of Recommended Biosafety Levels for Infectious Agents

Summary of Recommended Biosafety Levels for Infectious Agents

Bio-Safety Level Agents Practices Safety Equipment
(Primary Barriers
)
Facilities
(Secondary Barriers)
1 Not known to cause disease in healthy adults. Standard Microbiological Practices None required Open bench-top sink required
2 Associated with human disease, hazard = autoinoculation, ingestion, mucous membrane exposure

BSL-1 practice plus:

  • Limited access
  • Biohazard warning signs
  • Sharps precautions
  • Biosafety manual
    defining any needed waste decontamination
    or medical surveillance
    policies
Primary barriers = Class I or II BSCs or other physical containment devices used for all manipulations of agents that cause splashes or aerosols of infectious materials as well as PPE such as laboratory coats, gloves, face protection as needed BSL-1 plus ensure that an autoclave is available
3 Indigenous or exotic agents with potential for aerosol transmission; disease may have serious or lethal consequences.

BSL-2 practice plus:

  • Controlled access
  • Decontamination of all waste
  • Decontamination of lab clothes before laundering
Primary barriers = Class I or II BSCs or other physical containment devices used for all manipulations of agents as well as PPE such as protective lab clothing, gloves and respiratory protection, as needed

BSL-2 plus:

  • Physical separation from access corridors
  • Self-closing, double door access
  • Exhaust air not recirculated
  • Negative airflow into laboratory
 

This table was taken from the 5th Edition of the "Biosafety in Microbiological and Biomedical Laboratories" Handbook.